HTT 202 Histology Internship


Campus Location:
Wilmington
Effective Date:
2020-51
Prerequisite:
HTT 201, HTT 212, HTT 221, ENG 102
Co-Requisites:

None

Course Credits and Hours:
9.00 credits
1.00 lecture hours/week
24.00 lab hours/week
Course Description:

This supervised internship provides students with the opportunity to perform and refine all basic and specialized procedures in a histology laboratory setting.

Required Text(s):

Obtain current textbook information by viewing the campus bookstore online or visit a campus bookstore. Check your course schedule for the course number and section.

Additional Materials:

Histotechnician Program Manual

Allied Health/Science Department Student Policy Manual

Schedule Type:
Classroom Course
Disclaimer:

None

Core Course Performance Objectives (CCPOs):
  1. Demonstrate performance of accessioning and gross-room activities. (CCC 1, 2, 3, 4, 5, 6; PGC 1, 2, 6, 8, 9, 10, 12)
  2. Apply, demonstrate, and evaluate tissue processing procedures. (CCC 2, 5; PGC 2, 5, 6, 7, 8, 12)
  3. Demonstrate proper embedding techniques of tissues. (CCC 2; PGC 2, 5, 6, 7, 8, 10, 12)
  4. Demonstrate and evaluate microtomy proficiency. (CCC 2; PGC 2, 5, 6, 7, 8, 10, 12)
  5. Demonstrate and assess performance of routine hematoxylin and eosin (H&E) staining techniques. (CCC 2, 5, 6; PGC 2, 4, 5, 6, 7, 8, 12)
  6. Demonstrate appropriate slide preparation and sorting for pathologist review. (CCC 2, 4, 5, 6; PGC 1, 2, 4, 5)
  7. Discuss and demonstrate performance of selected special staining techniques by manual and automated methods. (CCC 2, 5, 6; PGC 2, 4, 5, 6, 7, 8, 12)
  8. Describe and demonstrate the procedures for receiving and performing frozen sectioning technique. (CCC 3, 4; PGC 2, 3, 4, 5, 8, 12)
  9. Assess and discuss the importance of maintaining professional conduct in all internship situations. (CCC 1, 3, 4, 5; PGC 8, 9, 10, 11, 12)
  10. Demonstrate preparing tissue, producing quality slides, and organizing laboratory resources and personnel. (CCC 1, 2, 3, 4, 5, 6; PGC 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)

See Core Curriculum Competencies and Program Graduate Competencies at the end of the syllabus. CCPOs are linked to every competency they develop.

Measurable Performance Objectives (MPOs):

Upon completion of this course, the student will:

  1. Demonstrate performance of accessioning and gross-room activities.
    1. Demonstrate receiving, accessioning, and charging of specimens using the laboratory information system.
    2. Demonstrate accurate specimen identification and handling of mislabeled specimens.
    3. Demonstrate the application methods to assist pathologists and pathologist assistant's with gross description.
    4. Apply proper type and length of fixation for various tissue types, including but not limited to, breast, testicular, gastrointestinal, and lymph node.
    5. Demonstrate proper handling and send-out procedures for outsourced services such as stones, lymph nodes for cell markers, renal biopsies, and muscle biopsies.
    6. Demonstrate proper decalcification procedures for small and large bone.
    7. Demonstrate appropriate disposal of chemical and surgical biohazardous materials.
    8. Monitor and document inventory supplies on a regular basis.
    9. Indicate and demonstrate proper personal protective equipment requirements and safety regulations.
  2. Apply, demonstrate, and evaluate tissue processing procedures.
    1. Demonstrate the principle and purpose of tissue processing.
    2. Demonstrate programming, operation, and maintenance of different tissue processors.
    3. Demonstrate manual processing of tissues.
    4. Demonstrate the processing of routine, stat, and brain specimens.
    5. Demonstrate the application method of reprocessing tissue.
    6. Evaluate and solve processing problems.
    7. Demonstrate processing quality control procedures.
    8. Comply with and demonstrate proper personal protective equipment requirements and safety regulations.
  3. Demonstrate proper embedding techniques of tissues.
    1. Demonstrate the purpose and principle of embedding.
    2. Demonstrate the application method of proper operation and maintenance of an embedding center.
    3. Apply and arrange tissues for proper orientation in molds (e.g., cervical cone, skin, and vas deferens).
    4. Demonstrate removal of molds and block preparation before cutting.
    5. Recognize and troubleshoot improper embedded tissues.
    6. Demonstrate proper quality control of an embedding center.
    7. Indicate and demonstrate proper personal protective equipment requirements and safety regulations.
  4. Demonstrate and evaluate microtomy proficiency.
    1. Demonstrate the purpose and principle of microtomy.
    2. Demonstrate proper care, operation, maintenance, and quality control of microtomes and flotation devices.
    3. Demonstrate proper microtomy technique for selected tissues.
    4. Produce acceptable tissue sections for microscopic evaluation.
    5. Evaluate and solve problems in tissue sectioning.
    6. Comply with and demonstrate proper personal protective equipment requirements and safety regulations.
  5. Demonstrate and assess performance of routine hematoxylin and eosin (H&E) staining techniques.
    1. Demonstrate the principle and purpose of routine H&E staining.
    2. Compare and demonstrate regressive and progressive staining—state the differences and similarities; advantages and disadvantages of each.
    3. Demonstrate the application method for each individual steps of routine and non-routine H&E staining using manual and automated staining methods.
    4. Demonstrate preparation and use of solutions and reagents for staining.
    5. Evaluate and solve staining problems.
    6. Demonstrate the application method used to coverslip stained slides.
    7. Evaluate and solve coverslipping artifact.
    8. Demonstrate coverslipping technique by manual and automated methods.
    9. Demonstrate proper usage and maintenance of an automated coverslipper.
    10. Demonstrate quality control/quality assurance of stained slides.
    11. Demonstrate proper personal protective equipment requirements and safety regulations.
  6. Demonstrate appropriate slide preparation and sorting for pathologist review.
    1. Arrange slides according to case numbers.
    2. Produce labels from the laboratory information system for slides.
    3. Demonstrate proper slide labeling and matching of the case to proper protocols for delivery to the pathologist.
    4. Demonstrate proper usage and maintenance of labeling equipment.
  7. Discuss and demonstrate performance of selected special staining techniques by manual and automated methods.
    1. Demonstrate the purpose and principle of various special stains by manual and automated methods.
    2. Distinguish acceptable and unacceptable staining results and troubleshoot staining problems.
    3. Discuss appropriate control tissue for various special stains, and identify organisms or entities being stained.
    4. Demonstrate chemical preparation, use, and storage of chemicals.
    5. Comply with disposal of chemicals, and monitor inventory of chemical supplies.
    6. Demonstrate the application process for immunohistochemistry staining techniques using manual and automated methods.
    7. Demonstrate proper usage and maintenance of an automated stainer.
    8. Demonstrate proper personal protective equipment requirements and safety regulations.
  8. Describe and demonstrate the procedures for receiving and performing frozen sectioning techniques.
    1. Demonstrate the purpose and principle of frozen sectioning.
    2. Identify cases for frozen sectioning.
    3. Demonstrate frozen section technique and staining of tissue sections within the allotted timeframe.
    4. Discuss the diagnostic limitations of frozen sectioning.
    5. Demonstrate proper cryostat operation and maintenance.
    6. Demonstrate proper personal protective equipment requirements and safety regulations.
  9. Assess and discuss the importance of maintaining professional conduct in all internship situations.
    1. Relate appropriate interpersonal skills with students, patients, visitors, and all other clinical, hospital, or college personnel.
      1. Demonstrate written, verbal, and non-verbal communication skills.
      2. Demonstrate punctuality in assignments and attendance.
      3. Demonstrate participation, initiative, and teamwork.
      4. Demonstrate problem-solving ability, time-management, and organizational skills in responding to laboratory pressures and stress.
      5. Accept constructive feedback.
      6. Modify interpersonal skills and professional behaviors during the internship.
      7. Given the core values of the clinical sites or affiliates, the student explains the need to demonstrate compliance.
    2. Self-assess and practice professional appearance and neatness.
    3. Given the Health Insurance Portability and Accountability Act (HIPAA) regulations for confidentiality, analyze appropriate practices.
    4. Demonstrate safety measures.
    5. Defend the importance of continued educational growth as a means of maintaining professional competence.
  10. Demonstrate preparing tissue, producing quality slides, and organizing laboratory resources and personnel.
    1. Select an assigned number of tissue types.
    2. Prepare tissue for processing and embedding.
    3. Produce quality tissue sections.
    4. Prepare the slides, for interpretation, using routine or special staining techniques.
    5. Prepare laboratory resources and personnel to manage time, the workload, , laboratory pressures, and stress to complete a comprehensive project within a given time frame.
Evaluation Criteria/Policies:

Students must demonstrate proficiency on all CCPOs at a minimal 75 percent level to successfully complete the course. The grade will be determined using the Delaware Tech grading system:

92 100 = A
83 91 = B
75 82 = C
0 74 = F

Students should refer to the Student Handbook for information on the Academic Standing Policy, the Academic Integrity Policy, Student Rights and Responsibilities, and other policies relevant to their academic progress.

Final Course Grade:

Calculated using the following weighted average

Evaluation Measure

Percentage of final grade

Midterm Exam: 1exam is weighted at 15% (summative)

15%

Final Exam: 1 exam weighted at 15% (summative)

15%

Capstone Slide Project (summative)

15%

Clinical Internship Checklist (summative)

30%

Enrichment Site Evaluation (formative)

15%

Generic Abilities-Affective Objectives Assessment (summative)

10%

TOTAL

100%

Core Curriculum Competencies (CCCs are the competencies every graduate will develop):
  1. Apply clear and effective communication skills.
  2. Use critical thinking to solve problems.
  3. Collaborate to achieve a common goal.
  4. Demonstrate professional and ethical conduct.
  5. Use information literacy for effective vocational and/or academic research.
  6. Apply quantitative reasoning and/or scientific inquiry to solve practical problems.
Program Graduate Competencies (PGCs are the competencies every graduate will develop specific to his or her major):
  1. Receive and accession tissue specimens accurately.
  2. Prepare tissue specimens for microscopic examinations, including all routine procedures.
  3. Assist with frozen section procedures in histopathology.
  4. Identify tissue structures and their staining characteristics.
  5. Perform preventive and corrective maintenance of equipment and instruments or refer to appropriate sources for repairs.
  6. Explain factors that affect procedures and results, and take appropriate action within predetermined limits when corrections are indicated.
  7. Perform and monitor quality control within predetermined limits.
  8. Apply principles of safety to all clinical laboratory procedures.
  9. Demonstrate professional conduct and interpersonal communications skills with patients, the public, laboratory and other health care personnel.
  10. Describe the responsibilities of other laboratory and health care personnel and interact with them with respect for their jobs and patient care.
  11. Explain and act upon individual needs for continuing education as a function of growth and maintenance of professional competence.
  12. Exercise principles of management, safety and supervision within the clinical laboratory environment.
Disabilities Support Statement:

The College is committed to providing reasonable accommodations for students with disabilities. Students are encouraged to schedule an appointment with the campus Disabilities Support Counselor to request an accommodation needed due to a disability. A listing of campus Disabilities Support Counselors and contact information can be found at the disabilities services web page or visit the campus Advising Center.

Minimum Technology Requirements:
Minimum technology requirements for online, hybrid, video conferencing and web conferencing courses.